NOVEL RNAi TECHNOLOGIES

The Multivalent RNA molecule (MV-RNA) represent a new RNAi trigger for the treatment of disease. The non-canonical structure utilizes the same RISC pathway as siRNA, but expands gene targeting, eliminates sense-strand off-target effects, and introduces the first 'junction containing' structure to RNAi. With an exlusive IP position and novel technical characteristics, Halo-Bio is working to release Multivalent RNAi based products that utilize MV-RNA's unique capabilities.

EXPERTS IN SINGLE-MOLECULE, MULTI-GENE SUPPRESSION

Multivalent RNA is an elegant single-molecule with advantages in molecular characterization, product cost, and IP ROI. While MV-RNA are more complicated than siRNA given their multivalency, Halo has the expertise and tools to apply MV-RNA to just about any series of target genes.

SOPHISTICATED DESIGN, POWERFUL API, ELEGANT DELIVERY

Halo has spent years developing design support in the form a a powerful computational array called, "Multivalent RNAi Cloud". This enterprise system ensures that products using MV-RNA as the Active Pharmaceutical Ingredient are based on the best possible designs for efficacy and specificity. MV-RNA share the same mechanism of action with canonical siRNA, but they are simply different otherwise. Knowing how to take advantage of this difference is central to Halo being able to provide novel product design, function, and even a new form of RNAi delivery.

DESIGN-TO-DRUG SCREENING

RNAi Cloud provides simplified MV-RNA design with real-time RNA folding, multi-target alignments, organism BLAST, nucleotide editing, RISC-loading energetics, target visualization, and predictive efficacy- all with research grade or GMP manufacturing support.

MULTI-GENE RNAi PROJECTS

RNAi Cloud stores your private projects as well as pre-designed projects targeting pathways in Cancer, Metabolic Diseases, Virus, and more.

"RNAi CLOUD" iOS APP

The complexities of Multivalent RNAi design and project organization is controlled by an easy to use iOS App.

SCALABLE COMPUTATIONAL ARRAY

RNAi Cloud is backed by a fully scalable design providing enterprise-wide access for tens of thousands of researchers. RNAi Cloud can be accessed through Halo-Bio's servers, or through private installations at your institution.

MV-RNA COMPLEX

MV-RNA are composed of three RNA guide strands 21-24nt long. Unlike siRNA, which contain a passenger and a guide strand, MV-RNA are composed of 3 guide strands- each aligning to a potential target site.

DICER SUBSTRATE MV-RNA

Single-stranded MV-RNA are designed for applications where a precursor MV-RNA are beneficial to the product use. Uses in Bioagriculture, in-situ production of MV-RNA from a plasmid, or as a HALO PARTICLE module are examples where 'Dicer-Substrate MV-RNA' are advantageous. Single-stranded MV-RNA manufacturing by T7 transcription allows for a cost-effective production of mid and large-scale quantities of MV-RNA.

APTAMER TARGETED MV-RNA MODULE

MV-RNA Precursors with RNA aptamers enable cell-specific targeting of 'Dicer Substrate MV-RNA' either alone or contained with the structure of the HALO PARTICLE.

A LOOK AT SUPPRESSION: ApoB

Converting a known siRNA into a Univalent MV-RNA (one active, two inactive guide strands) results in a repeatable dose dependence with suppressing targets such as ApoB.

IMMUNE STIMULATORY PROFILE: IL-6

MV-RNA are capable of exhibiting a clean IL-6 immunological response without a loss of activity.

SMALL ENOUGH TO PENETRATE

Programmable precise size ranges from 20-100 nanometers, MV-RNA are small enough to accumulate at tumors and sites of inflammation by the EPR effect and could possibly lead to potent treatments for difficult-to-treat diseases.

LARGE ENOUGH TO CIRCULATE

Extending circulation enhances cell-specific targeting. HALO PARTICLES above 20 nanometers help avoid rapid renal clearance.

PROGRAMMABLE TARGETING

MV-RNA modules within a HALO PARTICLE contain RNA apatmers designed to bind to certain cell types like Cancer or HBV infected cells. MV-RNA can be programmed to bind to target cells for the therapeutic and avoid non-targeted healthy tissue.

BIOGENESIS OF MV-RNA MODULES

MV-RNA modules are processed by endonucleases in the cell to produce the MV-RNA Complex in-situ. Single-stranded MV-RNA pre-cursor modules exhibit unique characteristics allowing finite nanoarchitectures, thermodynamics, serum stability, and duration of RNAi.

ENDOSOMAL STOICHIOMETRY

HALO PARTICLES are designed to disassemble at endosomal pH to facilitate release of MV-RNA into the cell for biogenesis and subsequent RNAi activation.